Journal: Regenerative Therapy

Article Title: Mesenchymal stromal cell administration promotes macrophage-mediated bile duct regeneration

doi: 10.1016/j.reth.2026.101065

Figure Lengend Snippet: MSC treatment increases scar-healing macrophages during bile duct regeneration (A) Experimental outline for MSC treatment. (B–D) Histological and immunofluorescence images show broader CK19 + ductular expansion in mice receiving MSCs compared to controls. (E–G) MSC administration is associated with increased populations of Sox9 + and Ki67 + regenerative epithelial cells, indicating an enhanced biliary repair response.

Article Snippet: Human adipose tissue-derived MSCs (passage 2) were obtained from PromoCell (cat#: C-12977, Heidelberg, Germany) and expanded until passage 4 using StemPro MSC SFM XenoFree medium (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C.

Techniques: Immunofluorescence

Journal: Regenerative Therapy

Article Title: Mesenchymal stromal cell administration promotes macrophage-mediated bile duct regeneration

doi: 10.1016/j.reth.2026.101065

Figure Lengend Snippet: Macrophage depletion negates MSC treatment-induced improvements in bile duct regeneration (A) Schematic illustration of the experimental design, including MSC administration and clodronate liposome-mediated macrophage depletion. (B–D) Clodronate treatment in combination with MSCs resulted in marked body weight loss compared with MSC treatment alone. (E) Immunofluorescence imaging demonstrated that the depletion of macrophages attenuated MSC-associated bile duct regeneration, as shown by a reduction in CK19 + ductular structures and decreased Ki67 + proliferating cells within CK19 - regions. (F) Sox9 + cells within the CK19 - compartment were also diminished in the MSC + Clip group. (G) Active YAP + cells within CK19 + biliary areas were reduced following macrophage depletion, indicating that macrophages contribute to the MSC-associated enhancement of biliary regenerative signaling.

Article Snippet: Human adipose tissue-derived MSCs (passage 2) were obtained from PromoCell (cat#: C-12977, Heidelberg, Germany) and expanded until passage 4 using StemPro MSC SFM XenoFree medium (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C.

Techniques: Immunofluorescence, Imaging